1UBN
SELENOSUBTILISIN BPN
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 287 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 85.880, 74.450, 42.430 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.400 |
| R-factor | 0.202 |
| Rwork | 0.202 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2st1 |
| RMSD bond length | 0.060 |
| RMSD bond angle | 2.900 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 8.000 |
| High resolution limit [Å] | 2.500 |
| Number of reflections | 11589 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6 * | pH 7.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3-5 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 27 (%sat) | |
| 3 | 1 | drop | sodium acetate | 0.5 (M) | |
| 4 | 1 | drop | 10 (mM) | ||
| 5 | 1 | reservoir | ammonium sulfate | 40 (%sat) |
