1UBF
MsREcA-ATPgS complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 2002-02-16 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | P 61 |
Unit cell lengths | 103.275, 103.275, 73.200 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 14.930 - 3.500 |
R-factor | 0.217 |
Rwork | 0.217 |
R-free | 0.29300 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1g19 |
RMSD bond length | 0.392 |
RMSD bond angle | 3.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 3.620 |
High resolution limit [Å] | 3.500 | 3.500 |
Rmerge | 0.468 | |
Number of reflections | 5464 | |
<I/σ(I)> | 6.7 | 2.1 |
Completeness [%] | 95.7 | 89.7 |
Redundancy | 3.5 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG 4000, CIT-PHOS, NACL, DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |