1UBB
Crystal structure of rat HO-1 in complex with ferrous heme
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-D |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2003-03-21 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 65.300, 65.300, 120.500 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.200 - 2.300 |
R-factor | 0.211 |
Rwork | 0.185 |
R-free | 0.22000 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1ivj |
RMSD bond length | 0.006 |
RMSD bond angle | 1.141 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.200 | 2.420 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 13453 | |
<I/σ(I)> | 6.3 | 2.1 |
Completeness [%] | 97.7 | 100 |
Redundancy | 6.4 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | Sodium formate, Potassium phosphate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |