1UBB
Crystal structure of rat HO-1 in complex with ferrous heme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-D |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-03-21 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 65.300, 65.300, 120.500 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.200 - 2.300 |
| R-factor | 0.211 |
| Rwork | 0.185 |
| R-free | 0.22000 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1ivj |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.141 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.200 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 13453 | |
| <I/σ(I)> | 6.3 | 2.1 |
| Completeness [%] | 97.7 | 100 |
| Redundancy | 6.4 | 6.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | Sodium formate, Potassium phosphate, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
