1UB6

Crystal structure of Antibody 19G2 with sera ligand

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL11-1
Synchrotron site	SSRL
Beamline	BL11-1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	194.429, 60.536, 92.297
Unit cell angles	90.00, 117.30, 90.00

Refinement procedure

Resolution	19.430 - 2.120
Rwork	0.264
R-free	0.29200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	19.430
High resolution limit [Å]	2.120

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.5	277	PEG4000, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K