1UB2
Crystal structure of catalase-peroxidase from Synechococcus PCC 7942
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-12-07 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 109.208, 109.208, 202.661 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 14.980 - 2.400 |
| R-factor | 0.199 |
| Rwork | 0.199 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1itk |
| RMSD bond length | 0.015 |
| RMSD bond angle | 2.100 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.800 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.064 | 0.214 |
| Number of reflections | 46151 | |
| <I/σ(I)> | 9.7 | 3.4 |
| Completeness [%] | 95.5 | 95.5 |
| Redundancy | 3.6 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.3 | 277 | Sodium formate, pH 6.3, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
