1UAZ

Crystal structure of archaerhodopsin-1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2002-12-13
Detector	MARRESEARCH
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	128.100, 128.100, 117.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 3.400
Rwork	0.230
R-free	0.28190
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1iw6
RMSD bond length	0.008
RMSD bond angle	1.200
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	XTALVIEW

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	3.580
High resolution limit [Å]	3.400	3.400
Number of reflections	12632
<I/σ(I)>	6.5	1.3
Completeness [%]	90.4	99.9
Redundancy	12.5	14.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.2	280	ammonium sulfate, octylthioglucoside, sodium citrate, heptanetriol, sodium chloride , pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 280K
1	VAPOR DIFFUSION, SITTING DROP	5.2	280	ammonium sulfate, octylthioglucoside, sodium citrate, heptanetriol, sodium chloride , pH 5.2, VAPOR DIFFUSION, SITTING DROP, temperature 280K