1UAQ
The crystal structure of yeast cytosine deaminase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-06-03 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.71 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.113, 53.681, 68.076 |
| Unit cell angles | 90.00, 105.33, 90.00 |
Refinement procedure
| Resolution | 50.000 * - 1.600 |
| R-factor | 0.177 |
| Rwork | 0.177 |
| R-free | 0.21500 |
| Structure solution method | MAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.600 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.066 | 0.338 * |
| Total number of observations | 277542 * | |
| Number of reflections | 42187 | 3945 * |
| <I/σ(I)> | 11.9 | 2.9 |
| Completeness [%] | 96.7 | 91.2 |
| Redundancy | 6.6 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | Hsu, Y.-H., (2003) Acta Crystallogr., Sect.D, 59, 950. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 2-propanol | 10 (%) | |
| 2 | 1 | reservoir | PEG4000 | 20 (%) | |
| 3 | 1 | reservoir | sodium HEPES | 0.1 (M) | pH7.5 |
| 4 | 1 | drop | protein | 30 (mg/ml) | |
| 5 | 1 | drop | 2-hydroxypyrimidine | 0-20 (mM) |
