1UAE
STRUCTURE OF UDP-N-ACETYLGLUCOSAMINE ENOLPYRUVYL TRANSFERASE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-12-09 |
| Detector | MARRESEARCH |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 110.660, 110.660, 67.550 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 6.000 - 1.800 |
| R-factor | 0.185 |
| Rwork | 0.185 |
| RMSD bond length | 0.018 * |
| RMSD bond angle | 2.850 * |
| Data reduction software | MOSFLM |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.800 * |
| Rmerge | 0.080 |
| Number of reflections | 41471 |
| Completeness [%] | 95.0 |
| Redundancy | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 8.5 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | UDP-GlcNAc | 2.5 (mM) | |
| 3 | 1 | reservoir | tert-butanol | 25 (%) | |
| 4 | 1 | reservoir | 100 (mM) | ||
| 5 | 1 | reservoir | glycerol | 10 (%) | |
| 6 | 1 | reservoir | Tris-HCl | 100 (mM) |
