1UA7
Crystal Structure Analysis of Alpha-Amylase from Bacillus Subtilis complexed with Acarbose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU ULTRAX 18 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2001-02-01 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.320, 74.204, 115.724 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.970 - 2.210 |
R-factor | 0.208 |
Rwork | 0.208 |
R-free | 0.26500 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.006 |
RMSD bond angle | 1.200 |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.490 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.097 | 0.239 |
Number of reflections | 31194 | |
<I/σ(I)> | 22.9 | 7.7 |
Completeness [%] | 93.7 | 93.7 |
Redundancy | 3.46 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | PEG 3350, calcium chloride, Tris-HCl, acarbose, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |