1UA3
Crystal structure of the pig pancreatic a-amylase complexed with malto-oligosaccharides
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 298 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-04-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 69.781, 113.183, 116.924 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.000 * - 2.010 |
| R-factor | 0.1835 |
| Rwork | 0.175 |
| R-free | 0.19800 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 1.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 * | 2.040 |
| High resolution limit [Å] | 2.010 | 2.010 |
| Rmerge | 0.055 | 0.218 |
| Total number of observations | 211556 * | |
| Number of reflections | 61415 * | |
| <I/σ(I)> | 9.5 | 1.6 |
| Completeness [%] | 98.3 | 85.8 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.8 * | 4 * | White, J.L., (1982) FEBS Lett., 148, 87. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 5 (mg/ml) | |
| 2 | 1 | drop | potassium phosphate | 50 (mM) | |
| 3 | 1 | drop | EDTA | 5 (mM) | |
| 4 | 1 | drop | DTE | 1-2 (mM) | |
| 5 | 1 | drop | PEG10000 | 16-18 (%(w/v)) | |
| 6 | 1 | reservoir | PEG10000 | 32-36 (%(w/v)) |
