1U9Y
Crystal Structure of Phosphoribosyl Diphosphate Synthase from Methanocaldococcus jannaschii
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-05-07 |
Detector | MARRESEARCH |
Wavelength(s) | 0.9794 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 71.011, 138.280, 138.360 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.650 |
R-factor | 0.221 |
Rwork | 0.220 |
R-free | 0.24200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Bacillus subtilis PRPP synthase |
RMSD bond length | 0.008 |
RMSD bond angle | 1.336 |
Data reduction software | DENZO |
Data scaling software | SCALA |
Phasing software | AMoRE |
Refinement software | CNS (0.9) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.700 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.081 | 0.415 |
Number of reflections | 40242 | |
<I/σ(I)> | 20.7 | 4.1 |
Completeness [%] | 99.8 | 99.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293 | PEG 4000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |