1U9Y

Crystal Structure of Phosphoribosyl Diphosphate Synthase from Methanocaldococcus jannaschii

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Detector technology	CCD
Collection date	2002-05-07
Detector	MARRESEARCH
Wavelength(s)	0.9794
Spacegroup name	P 21 21 21
Unit cell lengths	71.011, 138.280, 138.360
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.650
R-factor	0.221
Rwork	0.220
R-free	0.24200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Bacillus subtilis PRPP synthase
RMSD bond length	0.008
RMSD bond angle	1.336
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.700
High resolution limit [Å]	2.650	2.650
Rmerge	0.081	0.415
Number of reflections	40242
<I/σ(I)>	20.7	4.1
Completeness [%]	99.8	99.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.6	293	PEG 4000, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K