1U9R

Crystal Structure of Staphylococcal Nuclease mutant V66E/P117G/H124L/S128A at Room Temperature

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	2003-07-01
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 41
Unit cell lengths	48.794, 48.794, 63.788
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.790 - 2.100
R-factor	0.193
Rwork	0.193
R-free	0.21700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Staphylococcal nuclease V66E/P117G/H124L/S128A low-temperature coordinates
RMSD bond length	0.005
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.180
High resolution limit [Å]	2.100	2.100
Rmerge	0.072	0.215
Number of reflections	8569
<I/σ(I)>	28.8	10
Completeness [%]	97.7	96
Redundancy	4.5	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.4	277	MPD, potassium phosphate, pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K