1U9K
Crystal Structure of Mouse Triggering Receptor Expressed on Myeloid Cells 1 (TREM-1) at 1.76
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL11-1 |
| Synchrotron site | SSRL |
| Beamline | BL11-1 |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2004-06-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97954 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.837, 49.081, 125.208 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.470 - 1.760 |
| R-factor | 0.22614 |
| Rwork | 0.225 |
| R-free | 0.24841 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1smo |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.675 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.470 | 1.790 |
| High resolution limit [Å] | 1.760 | 1.760 |
| Number of reflections | 29567 | |
| <I/σ(I)> | 39.8 | 2.5 |
| Completeness [%] | 98.3 | 87.2 |
| Redundancy | 6.3 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 277 | MES, PEG 8000, zinc acetate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
