1U9I

Crystal Structure of Circadian Clock Protein KaiC with Phosphorylation Sites

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 5ID-B
Synchrotron site	APS
Beamline	5ID-B
Temperature [K]	108
Detector technology	CCD
Collection date	2002-07-10
Detector	MARRESEARCH
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	132.873, 135.576, 204.951
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.800
R-factor	0.25
Rwork	0.250
R-free	0.29000
Structure solution method	SAD
Starting model (for MR)	1tf7
RMSD bond length	0.010
RMSD bond angle	1.456
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE (RAVE)
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.043	0.022
Number of reflections	87750
<I/σ(I)>	20.6
Completeness [%]	99.6	99.3
Redundancy	9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION, RECRYSTALLIZATION	4	291	sodium formate, glycerol, pH 4, EVAPORATION, RECRYSTALLIZATION, temperature 291K
1	EVAPORATION, RECRYSTALLIZATION	4	291	sodium formate, glycerol, pH 4, EVAPORATION, RECRYSTALLIZATION, temperature 291K
1	EVAPORATION, RECRYSTALLIZATION	4	291	sodium formate, glycerol, pH 4, EVAPORATION, RECRYSTALLIZATION, temperature 291K