1U9G

Heterocyclic Peptide Backbone Modification in GCN4-pLI Based Coiled Coils: Replacement of K(8)L(9)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2004-04-13
Detector	RIGAKU RAXIS IV
Wavelength(s)	1.5418
Spacegroup name	P 41 3 2
Unit cell lengths	78.228, 78.228, 78.228
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.170 - 2.200
R-factor	0.24856
Rwork	0.245
R-free	0.32575
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.021
RMSD bond angle	2.664
Data reduction software	CrystalClear ((MSC/RIGAKU))
Data scaling software	CrystalClear ((MSC/RIGAKU))
Phasing software	PHASER
Refinement software	REFMAC (5.2.0003)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.170	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.031	0.326
Number of reflections	4526
<I/σ(I)>	11	2.2
Completeness [%]	100.0	98.8
Redundancy	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.4 M lithium sulfate monohydrate, 12% w/v PEG 8000, 20% v/v anhydrous glycerol, VAPOR DIFFUSION, HANGING DROP, temperature 295K