1U9A
HUMAN UBIQUITIN-CONJUGATING ENZYME UBC9
Experimental procedure
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7B |
Temperature [K] | 281 |
Detector technology | IMAGE PLATE |
Collection date | 1996-05-26 |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.040, 35.180, 58.100 |
Unit cell angles | 90.00, 111.20, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.000 |
R-factor | 0.16 * |
Rwork | 0.160 |
R-free | 0.25500 * |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1AAK |
RMSD bond length | 0.012 |
RMSD bond angle | 1.289 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | TNT (5E) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.120 | 0.420 |
Number of reflections | 12295 | |
<I/σ(I)> | 8.9 | 3.8 |
Completeness [%] | 91.0 | 94 |
Redundancy | 3 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion, hanging drop * | 7.5 | PROTEIN WAS CRYSTALLIZED IN THE SPACE GROUP P21 FROM 9% PEG 4000, 9% ISOPROPANOL, 0.1 M HEPES, PH 7.5 SYMMETRY OPERATIONS FOR NON-STANDARD SETTING: P 21: X, Y,Z -X,Y+1/2,-Z |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 11 (mg/ml) | |
2 | 1 | drop | 150 (mM) | ||
3 | 1 | drop | DTT | 0.5 (mM) | |
4 | 1 | drop | HEPES | 10 (mM) | |
5 | 1 | reservoir | PEG4000 | 9 (%) | |
6 | 1 | reservoir | isopropanol | 9 (%) | |
7 | 1 | reservoir | HEPES | 0.1 (M) |