1U99

Crystal Structures of E. coli RecA in a Compressed Helical Filament Form 4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Detector	SBC-2
Wavelength(s)	1.033
Spacegroup name	P 61
Unit cell lengths	108.001, 108.001, 74.410
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.180 - 2.600
Rwork	0.213
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB CODE 2REB
RMSD bond length	0.006
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.180	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.071	0.823
Number of reflections	14222
<I/σ(I)>	50.9	3.8
Completeness [%]	98.7	98.1
Redundancy	10.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	295	PEG 200, sodium citrate, sodium chloride, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K