1U98

Crystal Structure of E. coli RecA in a Compressed Helical Filament Form3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Detector	SBC-2
Spacegroup name	P 61
Unit cell lengths	108.391, 108.391, 73.783
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	31.290 - 2.000
Rwork	0.200
R-free	0.22000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PDB ID 2REB
RMSD bond length	0.005
RMSD bond angle	1.100
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	31.290	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.060	0.735
Number of reflections	33112
<I/σ(I)>	63.3	4.9
Completeness [%]	98.8	99.5
Redundancy	11.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.4	295	PEG 2000 Monomethylether, lithium sulfate, sodium phosphate, sodium citrate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 295K