1U98
Crystal Structure of E. coli RecA in a Compressed Helical Filament Form3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | SBC-2 |
Spacegroup name | P 61 |
Unit cell lengths | 108.391, 108.391, 73.783 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 31.290 - 2.000 |
Rwork | 0.200 |
R-free | 0.22000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ID 2REB |
RMSD bond length | 0.005 |
RMSD bond angle | 1.100 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.290 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.060 | 0.735 |
Number of reflections | 33112 | |
<I/σ(I)> | 63.3 | 4.9 |
Completeness [%] | 98.8 | 99.5 |
Redundancy | 11.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 295 | PEG 2000 Monomethylether, lithium sulfate, sodium phosphate, sodium citrate, pH 5.4, VAPOR DIFFUSION, HANGING DROP, temperature 295K |