1U94
Crystal Structure of E. Coli RecA in a Compressed Helical Filament Form 2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-07-30 |
Detector | SBC-2 |
Spacegroup name | P 61 |
Unit cell lengths | 105.854, 105.854, 73.496 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.650 - 1.900 |
Rwork | 0.187 |
R-free | 0.22200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2reb |
RMSD bond length | 0.004 |
RMSD bond angle | 1.200 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 34.650 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.079 | 0.713 |
Number of reflections | 35211 | |
<I/σ(I)> | 36.3 | 3.6 |
Completeness [%] | 99.8 | 100 |
Redundancy | 11.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 295 | PEG 550 MME, calcium chloride, hepes, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |