1U8Y

CRystal structures of Ral-GppNHp and Ral-GDP reveal two novel binding sites that are also present in Ras and Rap

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Wavelength(s)	1.0
Spacegroup name	P 42 2 2
Unit cell lengths	77.318, 77.318, 116.134
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.930 - 1.550
Rwork	0.213
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.100
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	34.600
High resolution limit [Å]	1.500
Number of reflections	51614

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291	20% PEG 3350, 0.2 M Magnesium Formate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K