1U8T
Crystal structure of CheY D13K Y106W alone and in complex with a FliM peptide
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-11-26 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.98397,0.97938,0.97925, 0.97239 |
| Spacegroup name | P 1 |
| Unit cell lengths | 54.280, 53.480, 54.100 |
| Unit cell angles | 60.36, 60.75, 60.57 |
Refinement procedure
| Resolution | 13.600 - 1.500 |
| R-factor | 0.2 |
| Rwork | 0.196 |
| R-free | 0.27300 |
| Structure solution method | MAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 1.325 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | SHARP |
| Refinement software | TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 13.600 | 1.510 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.100 | 0.374 |
| Number of reflections | 71506 | |
| <I/σ(I)> | 8.6 | 1.6 |
| Completeness [%] | 92.7 | 72 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.9 | 277 | PEG 3350, ammonium sulfate, Tris, VAPOR DIFFUSION, temperature 277K, pH 7.90 |
