1U8S

Crystal structure of putative glycine cleavage system transcriptional repressor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Detector	BRANDEIS - B4
Wavelength(s)	1.7
Spacegroup name	H 3
Unit cell lengths	145.022, 145.022, 51.506
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.450
R-factor	0.222
Rwork	0.222
R-free	0.26600
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.380
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SOLVE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.540
High resolution limit [Å]	2.450	2.450
Rmerge	0.053	0.340
Number of reflections	14865
<I/σ(I)>	15.5
Completeness [%]	99.9	99.7
Redundancy	4.6	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	293	Ammonium Sulfate, MES, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K
1	VAPOR DIFFUSION, SITTING DROP	6	293	Ammonium Sulfate, MES, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K