1U83
PSL synthase from Bacillus subtilis
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 150 |
Detector technology | CCD |
Collection date | 2004-07-29 |
Detector | CUSTOM-MADE |
Wavelength(s) | 0.97945, 0.97921 |
Spacegroup name | P 21 3 |
Unit cell lengths | 92.071, 92.071, 92.071 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.040 - 2.200 |
R-factor | 0.1922 |
Rwork | 0.188 |
R-free | 0.21900 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 1.400 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SHELXD |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 46.040 | 2.280 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 13466 | |
<I/σ(I)> | 12.9 | 3.17 |
Completeness [%] | 99.8 | 100 |
Redundancy | 10.6 | 5.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7 | 298 | Ammonium phosphate, Bis-Tris, ethylene glycol, glycerol, pH 7.0, VAPOR DIFFUSION, temperature 298K |