1U7R
Crystal structure of Native Sperm Whale myoglobin from low ionic strength enviroment (Form2 )
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-BM-D |
Synchrotron site | APS |
Beamline | 14-BM-D |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-01-01 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 39.950, 47.974, 78.332 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.000 - 1.150 |
R-factor | 0.1376 |
Rwork | 0.138 |
R-free | 0.16470 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.019 |
RMSD bond angle | 0.035 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SHELX |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.190 |
High resolution limit [Å] | 1.150 | 1.150 |
Rmerge | 0.039 | 0.235 |
Number of reflections | 53703 | |
<I/σ(I)> | 2.8 | |
Completeness [%] | 100.0 | 92.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | 50mM imidazole, pH 7 with 16% PEG3550, VAPOR DIFFUSION, HANGING DROP, temperature 277K |