1U7N
Crystal Structure of the fatty acid/phospholipid synthesis protein PlsX from Enterococcus faecalis V583
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-07-22 |
Detector | SBC-2 |
Wavelength(s) | 0.9793 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 62.362, 82.423, 147.237 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 73.520 - 2.260 |
R-factor | 0.2089 |
Rwork | 0.206 |
R-free | 0.26178 |
Structure solution method | SAD |
RMSD bond length | 0.013 |
RMSD bond angle | 1.443 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.2.0000) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 73.520 | 2.340 |
High resolution limit [Å] | 2.260 | 2.260 |
Rmerge | 0.127 | 0.640 |
Number of reflections | 36201 | |
<I/σ(I)> | 5.4 | 2.1 |
Completeness [%] | 99.4 | 94.6 |
Redundancy | 10.6 | 6.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 295 | Ethanol, HEPES, MgCl, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 295K |