1U7D
crystal structure of apo M. jannashii tyrosyl-tRNA synthetase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-08-05 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.119, 185.292, 95.482 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.680 - 2.650 |
R-factor | 0.229 |
Rwork | 0.227 |
R-free | 0.27700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1j1u |
RMSD bond length | 0.008 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.000 | 2.720 |
High resolution limit [Å] | 2.650 | 2.650 |
Number of reflections | 22575 | |
<I/σ(I)> | 21.5 | 2.1 |
Completeness [%] | 94.2 | 96 |
Redundancy | 3.5 | 3.12 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIQUID DIFFUSION | 5.5 | 277 | 12-15% PEG4000, 0.2M ammonium sulfate, 0.2M sodium acetate, , pH 5.5, LIQUID DIFFUSION, temperature 277K |