1U7B
Crystal structure of hPCNA bound to residues 331-350 of the flap endonuclease-1 (FEN1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-29 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9764 |
| Spacegroup name | P 63 |
| Unit cell lengths | 81.498, 81.498, 67.312 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 9.830 - 1.880 |
| R-factor | 0.217 |
| Rwork | 0.217 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 1AXC.pdb without chains B D F |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.920 |
| High resolution limit [Å] | 1.880 | 1.880 |
| Rmerge | 0.064 | 0.308 |
| Number of reflections | 20438 | |
| <I/σ(I)> | 43.5 | 6.42 |
| Completeness [%] | 98.4 | 96.6 |
| Redundancy | 9.27 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | ammonium sulfate, sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
