1U78
Structure of the bipartite DNA-binding domain of Tc3 transposase bound to transposon DNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-05-01 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.0749 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 93.681, 93.681, 255.558 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 12.000 - 2.690 |
| R-factor | 0.23515 |
| Rwork | 0.233 |
| R-free | 0.27291 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tc3 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.897 |
| Data reduction software | DENZO |
| Data scaling software | CCP4 ((TRUNCATE)) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.000 | 2.800 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Rmerge | 0.092 | |
| Number of reflections | 17820 | |
| <I/σ(I)> | 20 | 2 |
| Completeness [%] | 98.5 | 98.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | Na acetate, glycerol, DTT, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | Na acetate | ||
| 2 | 1 | 1 | glycerol | ||
| 3 | 1 | 1 | DTT | ||
| 4 | 1 | 2 | Na acetate | ||
| 5 | 1 | 2 | glycerol | ||
| 6 | 1 | 2 | DTT |
