1U76
Crystal structure of hPCNA bound to residues 452-466 of the DNA polymerase-delta-p66 subunit
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-29 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.9764 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 82.480, 82.480, 203.800 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.600 |
| R-factor | 0.24 |
| Rwork | 0.240 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB 1AXC.pdb without chains B D F |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.082 | 0.336 |
| Number of reflections | 24477 | |
| <I/σ(I)> | 7.4 | 3.8 |
| Completeness [%] | 96.0 | 100 |
| Redundancy | 4.36 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | ammonium sulfate, sodium acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
