1U6T
Crystal structure of the human SH3 binding glutamic-rich protein like
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2004-06-09 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 28.886, 34.815, 97.965 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.380 - 1.900 |
R-factor | 0.168 |
Rwork | 0.168 |
R-free | 0.21200 |
Structure solution method | SIRAS |
RMSD bond length | 0.005 |
RMSD bond angle | 1.200 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELXD (& SHARP) |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.380 | 1.990 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.050 | 0.120 |
Number of reflections | 8126 | |
<I/σ(I)> | 9.1 | 4.9 |
Completeness [%] | 98.0 | 85.2 |
Redundancy | 3.6 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | tri-Sodium Citrate dihydrate, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |