1U6H

Vinculin head (0-258) in complex with the talin vinculin binding site 2 (849-879)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	100
Detector technology	CCD
Collection date	2004-05-17
Detector	ADSC QUANTUM 4
Wavelength(s)	0.933
Spacegroup name	P 21 21 2
Unit cell lengths	52.122, 69.154, 96.162
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.380
R-factor	0.24574
Rwork	0.242
R-free	0.32230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	unpublished data
RMSD bond length	0.020
RMSD bond angle	2.115
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0003)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.442
High resolution limit [Å]	2.380	2.380
Rmerge	0.116
Number of reflections	14457
<I/σ(I)>	10.97
Completeness [%]	99.7	99.23

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	PEG 8000, Tris-HCl, magnesium chloride, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K