1U63
THE STRUCTURE OF A RIBOSOMAL PROTEIN L1-mRNA COMPLEX
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97895, 1.05 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 212.290, 68.900, 115.870 |
| Unit cell angles | 90.00, 122.99, 90.00 |
Refinement procedure
| Resolution | 8.000 - 3.400 |
| R-factor | 0.276 |
| Rwork | 0.276 |
| R-free | 0.30200 |
| Structure solution method | MAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 2.100 |
| Data reduction software | DENZO |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.610 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.085 | 0.435 |
| Number of reflections | 17645 | |
| <I/σ(I)> | 9.68 | 2.79 |
| Completeness [%] | 89.4 | 83.9 |
| Redundancy | 1.901 | 1.84 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 295 | PEG 10000, MPD, KCL, NACl, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 295K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 10000 | ||
| 10 | 1 | 2 | H2O | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | KCL | ||
| 4 | 1 | 1 | NACl | ||
| 5 | 1 | 1 | H2O | ||
| 6 | 1 | 2 | PEG 10000 | ||
| 7 | 1 | 2 | MPD | ||
| 8 | 1 | 2 | KCL | ||
| 9 | 1 | 2 | NACl |
