1U61
Crystal Structure of Putative Ribonuclease III from Bacillus cereus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-06-12 |
| Detector | SBC-2 |
| Wavelength(s) | 0.97930 |
| Spacegroup name | I 41 3 2 |
| Unit cell lengths | 168.978, 168.978, 168.978 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 2.150 |
| R-factor | 0.20729 |
| Rwork | 0.204 |
| R-free | 0.21640 |
| Structure solution method | SAD |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.467 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.200 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.099 | 0.690 |
| Number of reflections | 22642 | |
| <I/σ(I)> | 76 | 7.4 |
| Completeness [%] | 100.0 | 99.7 |
| Redundancy | 82 | 43.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 294 | Ammonium sulfate, cacodylate buffer, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
