1U60
MCSG APC5046 Probable glutaminase ybaS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97978 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.509, 155.891, 164.171 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.610 |
| R-factor | 0.14468 |
| Rwork | 0.143 |
| R-free | 0.17761 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.599 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.670 |
| High resolution limit [Å] | 1.610 | 1.610 |
| Number of reflections | 131904 | |
| Completeness [%] | 77.9 | 23 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | crystallization solution: 0.1M Hepes, 10% isopropanol, 20% PEG 4000. Cryoprotectant: crystallization solution plus 12% Ethylene glycol, pH 7.5 |
