1U5F
Crystal Structure of the PH Domain of SKAP-Hom with 8 Vector-derived N-terminal Residues
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-01-30 |
| Wavelength(s) | 0.916 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 78.750, 78.750, 130.123 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.22686 |
| Rwork | 0.226 |
| R-free | 0.24600 |
| Structure solution method | SIRAS |
| RMSD bond length | 0.031 |
| RMSD bond angle | 2.011 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.940 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.072 | 0.429 |
| Number of reflections | 11889 | |
| <I/σ(I)> | 19.6 | 2 |
| Completeness [%] | 95.2 | 97.7 |
| Redundancy | 7.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | Ammonium Sulfate, Sodium Citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
