1U5D
Crystal Structure of the PH domain of SKAP55
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 8-BM |
| Synchrotron site | APS |
| Beamline | 8-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-12-01 |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.068, 33.352, 105.382 |
| Unit cell angles | 90.00, 110.31, 90.00 |
Refinement procedure
| Resolution | 35.000 - 1.700 |
| R-factor | 0.168 |
| Rwork | 0.168 |
| R-free | 0.18500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Structure of the PH Domain of SKAP-Hom PDb entry 1u5g |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.800 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.040 | 0.183 |
| Number of reflections | 45007 | |
| <I/σ(I)> | 30 | 4 |
| Completeness [%] | 83.2 | 40.3 |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 298 | Ammonium Sulfate, Sodium Acetate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
