1U55
Crystal structure of an oxygen binding H-NOX domain related to soluble guanylate cyclases (oxygen complex)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-12-10 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.00 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 91.480, 126.990, 42.572 |
Unit cell angles | 90.00, 94.33, 90.00 |
Refinement procedure
Resolution | 29.970 - 1.770 |
Rwork | 0.227 |
R-free | 0.25700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1u4h |
RMSD bond length | 0.007 |
RMSD bond angle | 1.600 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.820 |
High resolution limit [Å] | 1.770 | 1.770 |
Number of reflections | 46008 | |
<I/σ(I)> | 26.4 | 3 |
Completeness [%] | 96.6 | 97.5 |
Redundancy | 2.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | PEG 3350, NaCl, pH 6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |