1U53
Novel X-Ray Structure of Na-ASP-2, a PR-1 protein from the nematode parasite Necator americanus and a vaccine antigen for human hookworm infection
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU FR-E SUPERBRIGHT |
Detector technology | IMAGE PLATE |
Collection date | 2003-12-16 |
Detector | RIGAKU RAXIS IV |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 37.890, 51.540, 43.630 |
Unit cell angles | 90.00, 114.06, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.560 |
R-factor | 0.17777 |
Rwork | 0.175 |
R-free | 0.22355 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.016 |
RMSD bond angle | 1.678 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.620 |
High resolution limit [Å] | 1.560 | 1.560 |
Rmerge | 0.154 | |
Number of reflections | 17644 | |
<I/σ(I)> | 27.6 | 3.5 |
Completeness [%] | 80.9 | 10.7 |
Redundancy | 1.74 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 100mM Imidazole, 40% PEG 400, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |