1U4S
Plasmodium falciparum lactate dehydrogenase complexed with 2,6-naphthalenedisulphonic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-09-14 |
| Detector | MACSCIENCE |
| Wavelength(s) | 1.5418 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 80.094, 86.096, 91.327 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.360 - 2.000 |
| R-factor | 0.13843 |
| Rwork | 0.136 |
| R-free | 0.18767 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB model 1LDG |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.481 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.150 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 19866 | |
| <I/σ(I)> | 13.6 | 4.1 |
| Completeness [%] | 90.9 | 79.5 |
| Redundancy | 6 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | MPD, Hepes, Imidazole, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
