1U4M
human RANTES complexed to heparin-derived disaccharide III-S
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | ENRAF-NONIUS FR571 |
Temperature [K] | 100 |
Detector technology | AREA DETECTOR |
Collection date | 2000-10-13 |
Detector | MARRESEARCH |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 24.074, 56.415, 94.354 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 18.440 - 2.000 |
Rwork | 0.200 |
R-free | 0.25200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1eqt |
RMSD bond length | 0.005 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNX (2002) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.970 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.047 | 0.164 |
Number of reflections | 10362 | |
<I/σ(I)> | 31.5 | 9.5 |
Completeness [%] | 96.0 | 90.9 |
Redundancy | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.5 | 293 | 15% (w/v) PEG 400, 100mM acetate buffer, 1mM Heparin Disaccharide III-S, 10% (w/v) glycerol, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |