1U4F

Crystal Structure of Cytoplasmic Domains of IRK1 (Kir2.1) channel

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2003-05-20
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0332
Spacegroup name	C 2 2 21
Unit cell lengths	112.461, 138.294, 138.745
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.440 - 2.410
Rwork	0.225
R-free	0.28100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1u4e
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	37871
<I/σ(I)>	18.47
Completeness [%]	88.0	90
Redundancy	3.31

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	isopropanol, Tris-HCl, MgCl2, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K