1U4E
Crystal Structure of Cytoplasmic Domains of GIRK1 channel
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-03-20 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.08 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 76.357, 76.357, 92.164 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 91.290 - 2.090 |
| R-factor | 0.19641 |
| Rwork | 0.194 |
| R-free | 0.23505 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n9p |
| RMSD bond length | 0.034 |
| RMSD bond angle | 2.378 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.150 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Number of reflections | 16100 | |
| <I/σ(I)> | 23.02 | |
| Completeness [%] | 94.5 | 73.32 |
| Redundancy | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | ethanol, hepes, magnesium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
