1U4C
Structure of spindle checkpoint protein Bub3
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 120 |
Detector technology | CCD |
Collection date | 2004-04-15 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97945, 0.97958, 0.9200 |
Spacegroup name | P 32 |
Unit cell lengths | 73.266, 73.266, 110.425 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.000 - 2.350 |
R-factor | 0.202 |
Rwork | 0.196 |
R-free | 0.28800 |
Structure solution method | MAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.440 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 2.390 |
High resolution limit [Å] | 2.350 | 2.350 |
Number of reflections | 52125 | |
<I/σ(I)> | 19 | 1.7 |
Completeness [%] | 94.6 | 93.2 |
Redundancy | 2.9 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 296 | 20% PEG 3350, 100mM citrate, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 296K |