1U42
Crystal structure of MLAM mutant of dimerisation domain of NF-kB p50 transcription factor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-02-23 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.864 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 59.403, 59.403, 59.856 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.260 - 2.699 |
| R-factor | 0.265 |
| Rwork | 0.259 |
| R-free | 0.31700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bfs |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.590 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.260 | 2.760 |
| High resolution limit [Å] | 2.699 | 2.699 |
| Rmerge | 0.347 | |
| Number of reflections | 3233 | |
| <I/σ(I)> | 17.5 | |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 292 | PEG 8000, ammonium sulphate, cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
