1U35
Crystal structure of the nucleosome core particle containing the histone domain of macroH2A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2003-02-27 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 105.505, 109.598, 175.989 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.000 |
| Rwork | 0.206 |
| R-free | 0.26040 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1aoi |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.020 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.095 | 0.421 |
| Number of reflections | 43366 | |
| <I/σ(I)> | 3.15 | |
| Completeness [%] | 99.5 | 100 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 292 | potassium chloride, manganese chloride, potassium cacodylate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 292K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | potassium chloride | ||
| 2 | 1 | 1 | manganese chloride | ||
| 3 | 1 | 1 | potassium cacodylate | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | potassium chloride | ||
| 6 | 1 | 2 | manganese chloride | ||
| 7 | 1 | 2 | potassium cacodylate | ||
| 8 | 1 | 2 | H2O |
