1U2M
Crystal Structure of Skp
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-06-08 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.9794, 0.9795, 0.9649 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.828, 84.190, 160.181 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 58.030 - 2.300 |
R-factor | 0.232 |
Rwork | 0.232 |
R-free | 0.25900 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.200 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 2.590 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 64535 | |
Completeness [%] | 99.6 | 99.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | PEG 350 monomethy ether, ammonium dihydrogen phosphate, Tris, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |