1U2K
Crystal structure of the C-terminal domain from the catalase-peroxidase KatG of Escherichia coli (I41)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-10-26 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.932 |
| Spacegroup name | I 41 |
| Unit cell lengths | 59.235, 59.235, 160.382 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 18.000 - 2.000 |
| R-factor | 0.19206 |
| Rwork | 0.189 |
| R-free | 0.24893 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1MWV |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.751 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.300 | 2.100 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 17719 | |
| Completeness [%] | 95.4 | 93.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 34% PEG 4000, 50 mM Sodium Acetate, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
