1U2H
X-ray Structure of the N-terminally truncated human APEP-1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.2 |
| Synchrotron site | BESSY |
| Beamline | 14.2 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2004-02-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9184 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 81.541, 25.522, 42.544 |
| Unit cell angles | 90.00, 104.59, 90.00 |
Refinement procedure
| Resolution | 5.000 - 0.960 |
| R-factor | 0.16113 |
| Rwork | 0.161 |
| R-free | 0.17213 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | SWISS MODEL (PDB ENTRIES 1FHG 1bih 1CS6) |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.782 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0003) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 0.980 |
| High resolution limit [Å] | 0.960 | 0.960 |
| Rmerge | 0.070 | 0.160 |
| Number of reflections | 46733 | |
| <I/σ(I)> | 29.9 | 4.5 |
| Completeness [%] | 89.1 | 47.3 |
| Redundancy | 3.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 30% PEG MME 2000, 200mM (NH4)2SO4, 100mM Na Acetate, pH 4.6, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
