1U2G

transhydrogenase (dI.ADPr)2(dIII.NADPH)1 asymmetric complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC QUANTUM 4
Wavelength(s)	0.934
Spacegroup name	P 21 21 21
Unit cell lengths	72.356, 73.766, 205.026
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.000 - 2.200
Rwork	0.222
R-free	0.25400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hzz
RMSD bond length	0.006
RMSD bond angle	1.181
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASES
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall
Low resolution limit [Å]	46.000
High resolution limit [Å]	2.200
Number of reflections	55412
<I/σ(I)>	17
Completeness [%]	100.0
Redundancy	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	ammonium sulphate, PEG, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K