1U2C

Crystal Structure of a-dystroglycan

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Detector technology	CCD
Spacegroup name	H 3
Unit cell lengths	71.445, 71.445, 144.233
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	35.720 - 2.300
R-factor	0.208
Rwork	0.208
R-free	0.26200
Structure solution method	MIR
RMSD bond length	0.007
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.720
High resolution limit [Å]	2.300	2.300
Number of reflections	11584
Completeness [%]	94.8	94.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1